Fig. 2

Inflammatory insult does not have a cytotoxic effect on mouse brain vascular pericytes but drives pericytes towards an inflammatory phenotype in vitro. A PDGFRβ expression levels after treatment with a combination of the three cytokines for 2 and 24 h. Pericyte lysates were analyzed by a PDGFRβ ELISA assay. B Pericytes were treated with each individual cytokine (100 ng/ml) or with a combination of the three cytokines for 2 and 24 h. The MTT assay was used to measure cytotoxicity. C Representative western blot images of pericytes treated with a combination of the three cytokines for 2 and 24 h. Bands were quantified with ImageLab and normalized to actin. D Total MMP9 levels in pericytes treated with a combination of the three cytokines for 2 and 24 h. Pericyte lysates were analyzed by a total MMP9 ELISA assay. All results are presented as mean ± SEM. *p < 0.05; **p < 0.01; *** p < 0.001; ****p < 0.0001 Multiple comparisons (ANOVA)