Fig. 6
From: Alpha-1 antitrypsin reduces inflammation and vasculopathy in mice with oxygen-induced retinopathy
AAT reduced retinal gliosis, VEGFA, and vascular leakage in phase II OIR mice at P18. RA, room air. Con, control. HSA, human serum albumin. AAT, alpha-1 antitrypsin. (A) Representative 3 μm paraffin sections of retina showing GFAP immunolabelling (green) in Müller cell processes (arrows). Nuclei counterstained with DAPI (blue). GCL, ganglion cell layer. IPL, inner plexiform layer. INL, inner nuclear layer. ONL, outer nuclear layer. Scale bar, 50 μm. (B) Quantitation of GFAP in the central, mid, peripheral and whole retina by One-way ANOVA. n = 5 to 6 mice per group. *p < 0.05, **p < 0.01, and ****p < 0.0001. C.Vegfa mRNA in retina. D. VEGFA protein in retina from mice perfused with PBS. E. Retinal vascular leakage (albumin ELISA) in retina from mice perfused with PBS. Values are mean ± SEM. n = 9 to 15 mice per group. *p < 0.05, ***p < 0.001, and ****p < 0.0001. Data were analysed by Welch ANOVA followed by Dunnett’s T3 tests and one-way ANOVA followed by Tukey’s test