Fig. 2
From: Alpha-1 antitrypsin reduces inflammation and vasculopathy in mice with oxygen-induced retinopathy
AAT reduced the number of Iba1 + cells and prevented the reduction in cell process length in phase II OIR mice at P18. RA, room air. Con, control. HSA, human serum albumin, AAT, alpha-1 antitrypsin. (A) Representative images of retinal flatmounts labelled with Iba1 showing microglia/macrophages (red, arrows), FITC-isolectin blood vessels (green), and DAPI nuclei (blue). Neovascular tufts are denoted by asterisks. Scale bar, 50 μm. (B) Quantitation of Iba1 + cells per mm2 within the ganglion cell layer and inner plexiform layer. (C) Quantitation of the primary cell process length of each Iba1+ cell within the ganglion cell layer and inner plexiform layer. n = 4 to 6 mice per group. Values are mean ± SEM. *p < 0.05, **p < 0.01, and ****p < 0.0001. Data were analysed by one-way ANOVA followed by Tukey’s test