Fig. 7
From: The injury effect of osteopontin in sepsis-associated lung injury
OPN-siRNA inhibits pyroptosis in MH-s cells treated with LPS. (A) The mRNA expression levels of NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. (B) Western blotting analysis of the relative expression of the NLRP3, GSDMD, caspase1, ASC, IL-1β and IL-18 in MH-s cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001. Osteopontin, OPN; NOD-, LRR- and pyrin domain-containing 3, NLRP3; Gasdermin D, GSDMD; apoptosis-associated speck-like protein containing a CARD, ASC; interleukin, IL; tumour necrosis factor, TNF; negative control, NC; Small interfering RNA, SiRNA; lipopolysaccharide, LPS; Mouse alveolar macrophage cells, MH-s; Quantitative Real-time reverse transcriptase-polymerase chain reaction, qPCR