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Fig. 5 | Journal of Inflammation

Fig. 5

From: The injury effect of osteopontin in sepsis-associated lung injury

Fig. 5

OPN was increased in LPS-treated MH-s cells, and OPN-siRNA could silence the expression of OPN. (A) The mRNA expression levels of OPN in MH-s cells were determined by qPCR. (B) ELISAs were used to detected the concentration of OPN in cell supernatant of MH-s cells. (C) The mRNA expression levels of OPN in MH-s cells were determined by qPCR. (D) ELISAs were used to detected the concentration of OPN in cell supernatant of MH-s cells. (E) Western blot analysis of the relative expression levels of OPN in MH-s cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001. Osteopontin, OPN; negative control, NC; Small interfering RNA, SiRNA; lipopolysaccharide, LPS; Mouse alveolar macrophage cells, MH-s; Quantitative Real-time reverse transcriptase-polymerase chain reaction, qPCR; enzyme-linked immunosorbent assay, ELISA

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Journal of Inflammation

ISSN: 1476-9255

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