Basement membranes’ role in immune cell recruitment to the central nervous system

Journal of Inflammation

Table 2 Shown are various mutations in mice in which core basement membrane proteins are knocked out, along with the associated phenotypes

Protein	CNS-resident cell expression	Mutation	Cre lines	Phenotypes	Ref.
Collagen 4A1	Endothelial cells Astrocytes Mesenchymal cells Pericytes [54,55,56,57]	Biallelic Δexon41 Monoallelic Δexon41 Conditional knockout	Tie2-Cre PDGFRβ-Cre GFAP-Cre	Embryonic lethality, intracerebral haemorrhage Perinatal lethality with intracerebral haemorrhage, porencephaly Intracerebral haemorrhage porencephaly Intracerebral haemorrhage porencephaly Mild intracerebral haemorrhage	[53] [31, 43] [52]
Collagen 4A1/4A2	Endothelial cells Astrocytes Mesenchymal cells Pericytes [54,55,56,57]	Missense mutations		Varying degrees of brain damage	[51, 53, 58]
Laminin α2	Astrocytes Oligodendrocytes Endothelial cells [59, 60]	Global null mutation		BBB disruption	[61, 62]
Laminin α4	Endothelial cells Oligodendrocyte precursor cells [60, 63]	Global null mutation		Perinatal haemorrhage	[64]
Laminin γ1	Astrocytes Endothelial cells Oligodendrocyte precursor cells [63, 65, 66]	Conditional knockout	Nestin-Cre PDGFRβ-Cre	BBB breakdown, intracerebral haemorrhage BBB breakdown and hydrocephalus on C57Bl6/FVB hybrid background Age-related mild BBB breakdown in C57BL6 background	[61, 67]

ISSN: 1476-9255