Fig. 6
From: Non-ionic surfactant vesicles exert anti-inflammatory effects through inhibition of NFκB
NISV alter inflammatory cytokines from BMDM following LPS, Poly(I;C), or Pam3csk4 stimulation. 100,000 BMDM were plated per well in triplicate and were stimulated using 3 µg/ml LPS, 10 µg/ml poly(I:C) and 320 ng/ml pam3csk4, these were then treated with 1.5 mM NISV. After 24 h supernatants were taken and analysed using a cytometric bead array. Heat map shows the Log2(fold change) of NISVcompared to their respective PAMP control. Statistical analysis was carried out using Prism 8 where a two-way ANOVA and Tukey’s multiple comparisons test was performed