Fig. 6

Circ_001653 stabilizes KEAP1 expression by recruiting BUD13. (A) Bioinformatic prediction of potential RNA-binding proteins (RBPs) that could bind to circ_001653. (B) Relative enrichment of circ_001653 in HK-2 cells reacted with different antibodies, detected by RIP assay. Anti-IgG served as the negative control. (C) Relative enrichment of KEAP1 in HK-2 cells treated with anti-BUD13, detected by RIP assay. Anti-IgG served as the negative control. (D) Interference efficiency of sh-BUD13-1/2/3 in HK-2 cells was determined using qRT-PCR. (E) Relative expression of KEAP1 in HK-2 cells transfected with sh-BUD13/NC was detected using qRT-PCR. sh-NC served as the negative control. (F) Relative expression of KEAP1 at 0, 6, 12, 18 and 24 h in HK-2 cells transfected with sh-BUD13/NC. sh-NC served as the negative control. (G) RIP assay detected the relative enrichment of KEAP1 in HK-2 cells treated with anti-BUD13/IgG and transfected with sh-circ_001653/NC. **P < 0.01, ***P < 0.001