Fig. 5

Circ_001653 silencing attenuates oxidative damage and inflammation in LPS-induced HK-2 cells by regulating KEAP1. Rescue assays were performed in HK-2 cells after transfection with sh-NC, sh-circ_001653, or sh-circ_001653 + oe-KEAP1 for 48 h and then treated with LPS (10 µg/mL) for 24 h. sh-NC was used as the negative control. (A) TUNEL assay was performed to measure apoptosis of HK-2 cells in corresponding groups. (B) The proliferation of HK-2 cells in the corresponding groups was detected by EdU assay. (C) Relative fluorescence levels of ROS in HK-2 cells from corresponding groups were detected by the DCFH-DA probe. (D) MDA, (E) SOD, (F) GSH activities and (G) CAT levels in the supernatant of HK-2 cells in indicated groups were detected using the corresponding kits. (H) The contents of IL-1β, IL-6 and TNF-α in the supernatant of HK-2 cells from corresponding groups were detected by ELISA. **P < 0.01, ***P < 0.001. Ctrl: Control; LPS: lipopolysaccharide; DAPI: 4’,6-diamidino-2-phenylindole; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling; EdU, Ethynyl-2ʹ-Deoxyuridine; ROS: reactive oxygen species; MDA: malondialdehyde; SOD: superoxide dismutase; GSH: glutathione; CAT: catalase; IL-1β: Interleukin (IL)-1beta; IL-6: Interleukin (IL)-6; TNF-α: tumor necrosis factor (TNF)-alpha; ELISA: Enzyme-Linked Immunosorbent Assay