Fig. 3
Circ_001653 inhibition alleviates renal dysfunction, oxidative stress, and inflammatory response in the CLP-induced SA-AKI rat model. Wistar rats were divided into the sham, CLP + sh-NC, and CLP + sh-circ_001653 groups (n = 6 per group). Rats in the CLP + sh-NC group or CLP + sh-circ_001653 group received ceacal ligation and puncture. The rats received injections of adenovirus carrying sh-NC or sh-circ_001653 via the tail vein. Rats in the sham group received caecum exposure without ligation. (A) qRT-PCR was performed to measure the relative expression level of circ_001653 in kidney tissues in indicated groups. (B) H&E staining was performed to evaluate the pathological morphology of renal tissues in indicated groups (left panel). A semi-quantitative histopathological score of renal tissue on a scale of 0-5 based on corresponding images (Right panel). Levels of (C) BUN and (D) sCr in serum from corresponding groups were detected using corresponding kits. Levels of urinary (E) NGAL and (F) KIM-1 were detected by ELISA. (G) Relative fluorescence levels of ROS in kidney tissues were detected by DCFH-DA staining. (H) Serum MDA levels in indicated groups were measured using the corresponding kit. Serum (I) SOD, (J) GSH activities, and (K) CAT levels in indicated groups were measured using the corresponding kits. Relative mRNA expression levels of (L) IL‐1β, (M) IL‐6, and (N) TNF‐α in kidney tissues were measured with quantitative PCR. (O) The serum levels of IL-1β, IL-6, and TNF-α in indicated groups were detected by ELISA. ***P < 0.001. CLP: cecal ligation and puncture; H&E: hematoxylin and eosin; BUN: blood urea nitrogen; sCr: serum creatinine; NGAL: Neutrophil gelatinase-associated lipocalin; KIM-1: Kidney Injury Molecule-1; MDA: malondialdehyde; SOD: superoxide dismutase; GSH: glutathione; CAT: catalase; IL-1β: Interleukin (IL)-1beta; IL-6: Interleukin (IL)-6; TNF-α: tumor necrosis factor (TNF)-alpha; ELISA: Enzyme-Linked Immunosorbent Assay