Fig. 1

Circ_001653 expression is high in the LPS-induced SA-AKI HK-2 cell model. (A) CCK-8 assay was used to detect the viability of HK-2 cells exposed to different concentrations (0, 2.5, 5, 10 µg/mL) of LPS for 24 h. (B) Relative expression level of circ_001653 in HK-2 cells stimulated with or without LPS at different concentrations (0, 2.5, 5, 10 µg/mL) for 24 h detected by qRT-PCR. (C) Relative expression level of circ_001653 detected at 0, 6, 12, and 24 h in HK-2 cells stimulated with 10 µg/mL LPS using qRT-PCR. (D) For RNase R digestion assay, HK-2 cells were treated with or without 3 U/µg RNase R for 1 h at 37 °C, and qRT-PCR was conducted to detect circ_001653 and its linear mRNA (DUSP22) expression in HK-2 cells. (E) Circ_001653 and linear mRNA (DUSP22) expression levels in HK-2 cells at different time points (0, 6, 12, 18, 24 h) after treatment with 2 mg/ml actinomycin D. (F-G) The localization of circ_001653 in HK-2 cells was detected by subcellular fractionation and FISH assays, respectively. **P < 0.01, ***P < 0.001. LPS: lipopolysaccharide; SA-AKI: sepsis-associated acute kidney injury; DUSP22: dual specificity phosphatase 22; DAPI: 4’,6-diamidino-2-phenylindole