Fig. 1

(A-P) Cross-sectional images of lesioned spinal cords in the Shh- and Control group 3- and 7-days post injury (dpi), stained for β-catenin (red) and Fibrinogen (blue) at different magnifications: 20x (A-D; scale bar: 150 μm), 10x (I-L; scale bar: 75 μm), and 10 × (4x4 tile-scans, E-H + M-P; scale bar: 500 μm). (Q) β-catenin staining (red) shows a significantly higher concentration in Shh-treated animals (n = 5) compared to untreated animals at 7 dpi (p = 0.0234, n = 6), as well as at 3 dpi (p = 0.0213). Blue staining highlights intramedullary evidence of Fibrinogen extravasation (I-P; blue). (R) While there was no significant difference in Fibrinogen accumulation between Shh-treated (n = 5) and untreated animals (n = 6) at 3 dpi, at 7 dpi, less Fibrinogen extravasation was observed in the Shh group (n = 5) compared to the Control group (p < 0.01, n = 6). Data are expressed as mean ± SEM pi/mm². Statistical analysis was conducted using one-way ANOVA with post hoc Tukey-HSD tests (*p < 0.05, **p < 0.01)