Table 1 Primary antibodies for western bolt
Primary Antibody | Catalog Number | Dilution Ratio | Source |
|---|---|---|---|
Gapdh | 60004-1-Ig | 1:50,000 | Proteintech Group, Wuhan, Hubei, China |
Aggrecan | 13880-1-AP | 1:1,000 | Proteintech Group, Wuhan, Hubei, China |
Collagen I | # 84,336 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
Collagen II | 28459-1-AP | 1:1,000 | Proteintech Group, Wuhan, Hubei, China |
Mmp2 | 66366-1-Ig | 1:1000 | Proteintech Group, Wuhan, Hubei, China |
Mmp3 | BM4074 | 1:500 | Boster, Wuhan, Hubei, China |
Mmp9 | 10375-2-AP | 1:500 | Proteintech Group, Wuhan, Hubei, China |
Mmp13 | 18165-1-AP | 1:1,000 | Proteintech Group, Wuhan, Hubei, China |
Cox-2 | # 12,282 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
Inos | #13,120 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
Il-6 | A14687 | 1:500 | ABclonal, Wuhan, Hubei, China |
Ppar-γ | A11183 | 1:1000 | ABclonal, Wuhan, Hubei, China |
p-P38 | #4511 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
P38 | #8690 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
p-Erk | #4377 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
Erk | #4695 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
p-Jnk | #4668 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
Jnk | #9252 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
p-P65 | #3033 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |
P65 | #8242 | 1:1,000 | Cell Signaling Technology, Beverly, MA, USA |