Fig. 5

Inhibition of Ppar-γ weakened the anti-inflammatory, anabolism enhancing, and catabolism suppressing effects of MA on IL-1β- stimulated NPCs. (A-B) The proteins expressions of Ppar-γ were analyzed with western blotting. (C-D) The expressions of anabolic proteins (Collagen II and Collagen I) and catabolic protein (Mmp13) in the Ppar-γ-inhibition NPCs along with or without the administration of 5 ng/ml of IL-1β and 80 µM of MA. (E) The mRNA expression of Collagen II, Mmp13, Cox-2, and Inos were detected by RT-qPCR analysis. Data were shown as means ± SD, n = 3. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, no significant difference