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Fig. 1 | Journal of Inflammation

Fig. 1

From: Mulberroside A mitigates intervertebral disc degeneration by inhibiting MAPK and modulating Ppar-γ/NF-κB pathways

Fig. 1

Viability of NPCs intervened with MA/IL-1β and choice of optimal concentrations of MA. (A) Chemical structure of MA. (B) The viability of NPCs intervened with different MA concentrations (0, 20, 40, 80, and 160 µM) for 24 h was evaluated by a CCK-8 kit. (C) The viability of NPCs was intervened with IL-1β (5 ng/mL) alone or with different MA concentrations (20, 40, and 80 µM) for 24 h was evaluated by a CCK-8 kit. (D) A Calcein /PI double staining of NPCs after intervention with IL-1β (5 ng/mL) alone or with different MA concentrations (20, 40, and 80 µM) for 24 h (green, living cells; red, nucleus of dead cells). (E) Quantitative analysis of the percentage of living cells. Data were shown as means ± SD, n = 3. ∗∗∗p < 0.001; ns, no significant difference

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Journal of Inflammation

ISSN: 1476-9255

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