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Fig. 4 | Journal of Inflammation

Fig. 4

From: Cinnamaldehyde inhibits the NLRP3 inflammasome by preserving mitochondrial integrity and augmenting autophagy in Shigella sonnei-infected macrophages

Fig. 4

Effect of CA on cathepsin B release in S. sonnei-infected macrophages. (A) LPS-primed J774A.1 macrophages were incubated with CA for 0.5 h before S. sonnei infection for an additional 21 h. The detectable levels of pro-cathepsin B and mature cathepsin B in the cell lysates (A) and in the supernatants (B) were analysed by Western blotting. The cathepsin B activity was analysed by Magic Red staining and acquired by fluorescent microscopy (C). The Western blotting images are representative of separate experiments. The fluorescent microscopy images are representative of different fields, while the histogram providing quantification of mean fluorescent intensity (MFI) expressed as the mean ± SD for these three fields. *p < 0.05 and ***p < 0.001 compared to S. sonnei-infected macrophages

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Journal of Inflammation

ISSN: 1476-9255

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