Fig. 3

IL-10 prevents LPS direct stimulation and TNF-preactivation of neutrophils by regulating PTM-modified enzymes. A Increases in expression of MLL1 and SETD1 proteins, being the result of direct LPS effect (left part of the graph), are neutralised by IL-10 (right part of each graph). The protective effect of IL-10 occurs only when IL-10 first acts on neutrophils. The upper panels presented average protein levels and low panel mRNA expression ± SD. * - statistically significant differences within: n.s.; TNF, LPS, IL-10 or TNF + LPS samples. ^# - statistically significant differences within IL-10 + TNF, TNF + 10, IL-10 + LPS or LPS + IL-10. A separate statistical comparison within protein levels is marked with a dashed line. B CHD1 is overexpressed during direct exposure of neutrophils to IL-10 or TNF, but not LPS. Co-stimulation of neutrophils with IL-10 and LPS disturbs CHD1 expression regardless of the order of LPS administration. C IL-10, opposite to LPS or TNF exposition, triggered JMJD2A