Fig. 4
From: Microcirculatory disturbance in acute liver injury is triggered by IFNγ-CD40 axis
Induction of tissue factor by IFNγ and CD40 ligand in liver endothelial cells. A The LSEC were treated with 1,000 U/ml IFNγ for 12 hours and then incubated for 6 hours with Jurkat cells fixed with 1% paraformaldehyde. B Cell-surface expression of CD40L protein in untreated Jurkat cells by flow cytometry. The histograms depict isotype control (dashed line), unstained control (solid blue line), and CD40L expression (solid red line). The qualitatively identical results were obtained with at least three further batches of cells. C RT-qPCR analysis of CD40 and tissue factor in LSEC cells after IFNγ (1,000 U/ml) and Jurkat cell treatment. The gene expression levels were normalized to those of the IFNγ and Jurkat cell free group. The data were expressed as mean and SE (n = 5–10 in each group). **P < 0.01 vs. the IFNγ and Jurkat cell free group and ††P < 0.01 vs. the IFNγ group. CD40L, CD40 ligand; IFNγ, interferon-gamma; LSEC, liver sinusoidal endothelial cell; RT-qPCR, quantitative reverse transcription polymerase chain reactions