Fig. 5

Akt signaling pathway mediates the pro-apoptosis role Crtc1 in ALI. (A-D) Western blot (A) and quantification (B-D) showing the role of Crtc1 gene manipulation on p-Akt/Akt. ^*P < 0.05, ^**P < 0.01 vs. Crtc1^+/+; ^#P < 0.05, ^###P < 0.001 vs. Sham; n = 3. (E, F) Representative TUNEL staining images (E) and quantification (F) showed Triciribine (10µM) blocked the inhibition of Crtc1 knockdown on LPS-induced TUNEL-positive cells. ^**P < 0.01 vs. LPS group; ^#P < 0.05 vs. siCrtc1 + LPS group; n = 3. (G-J) Western blot (G) and quantification (H-J) showing Triciribine reversed the protective effects of Crtc1 silencing on apoptosis-associated proteins, such as Cleaved-caspase3/Caspase3 (H), Bcl-2 (I) and Bax (J) in RAW264.7 cells. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. LPS group; ^#P < 0.05 vs. siCrtc1 + LPS group; n = 3. (K-N) Triciribine blocked the inhibition of Crtc1 knockdown on LPS-induced inflammation factors including TNF-α (K), IL-1β (L), IL-6 (M) and CXCL1 (N). ^*P < 0.05, ^***P < 0.001 vs. LPS group; ^#P < 0.05 vs. siCrtc1 + LPS group; n = 6