Fig. 2

β-FNAs effect on LPS-induced CXCL10 expression in the whole brain and brain regions (hippocampus, prefrontal cortex, and cerebellum/brain stem) of male and female C57BL/6J mice. Mice (n = 5–6/group) were injected (i.p.) with saline (control), LPS (0.83 mg/kg), LPS followed immediately by β-FNA treatment (50 mg/kg; i.p.; LPS + β-FNA), or LPS followed by β-FNA 10 h post-LPS (LPS + β-FNA 10 h). 24 h post-LPS, mice were terminated followed by tissue collection. Levels of CXCL10 in whole brain (A), hippocampus (B), prefrontal cortex (C), and cerebellum/brain stem (D) were measured by ELISA. Data are reported as mean ± SEM. Two-way ANOVA indicated a significant main effect of treatment (p < 0.0001) on CXCL10 levels in the whole brain; but no significant effect of sex (p = 0.27), nor a significant interaction (p = 0.88). Two-way ANOVA determined CXCL10 in the hippocampus had a significant main effect of sex (p < 0.01), treatment (p < 0.001), and interactions (p < 0.001). In the prefrontal cortex two-way ANOVA determined CXCL10 had a significant main effect of treatment (p < 0.0001) and interactions (p < 0.0001), but not sex (p = 0.94). Two-way ANOVA determined in the cerebellum/brain stem that CXCL10 had a significant main effect of treatment (p < 0.0001), and interactions (p < 0.01), but not sex (p = 0.73). Pairwise comparisons were assessed using a Fisher’s LSD test; * indicates p < 0.05 vs. saline group; # indicates p < 0.05 vs. LPS group