Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

Skip to main content
Fig. 3 | Journal of Inflammation

Fig. 3

From: Serinc2 deficiency causes susceptibility to sepsis-associated acute lung injury

Fig. 3

Serinc2 overexpression protects RAW264.7 cells from LPS-induced inflammation. A Validation of Serinc2 overexpression by qRT-PCR. RAW264.7 cells were transfected with Vector or Serinc2 plasmid (4 μg) for 48 h. ***P < 0.001 vs. Vector. n = 3. B Effects of Serinc2 overexpression on LPS-induced expression of IL1β, IL6, TNFα, and MCP1 in RAW264.7 cells. LPS (1 μg) was administrated for 4 h. **P < 0.01, ***P < 0.001 vs. Vector; ##P < 0.01, ###P < 0.001 vs. PBS. n = 3. C Time-dependent activation of STAT3, p38, and ERK pathways by LPS in RAW264.7 cells measured by Western blot. D Immunoblots showing the impact of Serinc2 overexpression on LPS-induced phosphorylation of STAT3, p38, and ERK. E Quantification of STAT3, p38 and ERK phosphorylation levels in ratio to their total protein. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Vector; ##P < 0.01, ###P < 0.001 vs. PBS. n = 3

Back to article page