Fig. 12

Human PBMC MTS viability assay. Human PBMC (1 × 106 cells/well) were cultured in serum-free media (37 °C, 5% CO2/95%O2 18 h) with LPS (50 ng/mL), SAGE, or vehicle alone. MTS tetrazolium compound was added and incubated for 2 h. The formazan dye was quantified by measuring the absorbance at 490 nm. Each value represents the mean of 3 cultures ± the standard error of the mean (S.E.M.)