Fig. 2

Overexpression of SP1 protected HK-2 cells against injury induced by anoxia-reoxygenation. The HK-2 cells were transfectd with SP1 overexpression vector. a-c RT-qPCR was used to detecte the expression of SP1, miR-205 and PTEN. d Western blot detected the protein expression of PTEN, AKT, p-AKT, Beclin-1,p62,LC3II and LC3I. e The autophagy protein LC3 expression localization was detected by immunofluorescence staining. f Cell proliferation was measured by MTT assay. g Flow cytometry analysis of cell apotosis. h-i The secretions of IL-6 and TNF-α were detected by ELISA. The data are expressed as the mean ± SD and are representative of 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001