Fig. 3

The anti-apoptotic effect of IL-35 in spinal cord tissues. Nissl staining to assess the loss of neurons (a) and spinal neuronal apoptosis detection using TUNEL assay (b) in Control, DNP, DNP + vehicle, SP, IL-35 and IL-35 + Ani groups, respectively. *P < 0.05 vs. the control group; #P < 0.05 vs. the DNP + vehicle group; $P < 0.05 vs. the IL-35 group. n = 6 per group