Fig. 1
From: The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation
N-glycosylation of CD200R1 and construction of CD200R1 mutant. a The CD200R1 expressed in BV2 cells in the presence of N-glycosylation inhibitors (1 μg/mL TM) for 24 h was determined by western blotting analysis. b Cell lysates of BV2 cells were denatured and treated with PNGaseF and subjected to western blotting analysis. c Cell lysates of BV2 cells were denatured and treated with endoglycosidase (Endo H) and subjected to western blotting analysis. d Four potential N-glycosylation sites at Asn35, Asn44, Asn192, Asn314 were presented. We mutated the asparagine residue (N44) to glutamine (Q) to verify the N-glycosylation site on CD200R1. e N44Q mutant was generated and transiently expressed in BV2 cells. Additionally, some of cell lysates were treated by enzymatic digestion and then subjected to western blotting analysis. Error bars indicate ± SEM