Figure 3
Effect of LBP on endotoxin-dependent IL-8 release by PBMC. (A) Freshly isolated human PBMC (~1.0 × 106 total cells/well, 100,000 monocytes) were prepared as described in Methods in 200 μl of incubation buffer with and without 100 ng/ml LBP, and with the indicated concentration of endotoxin. (B) PBMC and partially differentiated macrophages (~100,000 cells/well; see Methods) from the same donor were prepared in 200 μl of the same buffer containing 100 pg/ml of endotoxin and the indicated concentration of LBP. All cultures were then incubated for 6 h. At the end of the incubation period, samples were collected and frozen for subsequent IL-8 analysis by ELISA. Data are expressed as the mean ± S.E.M. of triplicate samples; error bars for some conditions were so small they are obscured by the symbols. (A) *p < 0.05, **p < 0.001 relative to the no LPS control. There were no significant differences between the presence and absence of LBP. (B) *p < 0.05 relative to the no LBP control. Similar results were seen in 3 independent experiments.