Figure 5
MTT and FCM analysis the effect of NK-1R antagonist to ASMC proliferation. In IL-13 intervention group ASMC proliferate more faster than in normal group, from 24 h to 48 h the difference become significant (P < 0.05). In NK-1R antagonist intervention group ASMC proliferate faster than in normal group but slower than in IL-13 intervention group, especially during 48 h to 72 h (Fig.5A). Flow cytometric analysis of ASMC cell cycle (Fig.5B, 5C, 5D). Most of ASMC stayed at G1 stage in WIN62577 intervention group compared with in normal group. B, C and D: representative examples of normal group, IL-13 intervention group and IL-13 with WIN62577 intervention group.