Figure 2
From: Fas (CD95) induces rapid, TLR4/IRAK4-dependent release of pro-inflammatory HMGB1 from macrophages

Fas activation for 24 hr does not affect viability of primary murine peritoneal mϕ. (A) Dose response analysis of viability of primary murine peritoneal mϕ treated with recombinant mFasL (0-0.5 μg/ml); (B) time course (0 - 24 hr) analysis of primary peritoneal mϕ following treatment with recombinant mFasL (0.5 μg/ml). Cell viability was determined by trypan blue exclusion using a cell viability analyzer as described in Materials and Methods. Results are presented as mean ± SD of 3 independent experiments, n = 3/group. * P ≤ 0.05; **P ≤ 0.01.