Figure 4

Effect of Pyk2 inhibition on spreading of dHL60 cells on plated fibrinogen. dHL-60 cells were preincubated with indicated concentrations of 2 μM TAT-Pyk2-CT (C) or TAT-GFP (D) for 15 min at 37°C, and adhered to plated fibrinogen for 30 min in the absence (A) or presence (B-D) of 1 μM fMLP. Cell morphology was analyzed by light microscopy. Representative images were shown from three experiments. Filled arrow points to lamellipodia, and empty arrow points to filopodia.