Figure 5

Cells spiked with MIF siRNA display reduced nuclear p21. In four separate experiments wt MDF nucleofected with MIF siRNA exhibited a significant decrease in nuclear p21 expression (b), while MDF nucleofected with control siRNA remained largely unchanged (a). The efficiency of MIF knockdown in wt MDF was quantified using RT-PCR. Compared with control siRNA, nucleofection of MIF siRNA leads to undetectable MIF mRNA in MDF as determined by RT-PCR (c) (N = 4, * P < 0.05).